The hydrodistilled essential oils of the flowers and leaves of Laserpitium zernyi by GC-FID and GC/MS. Fifty-five compounds were identified in the flower oil (96.8% of total oil) and 58 in the leaf oil (89.4% of total oil). Both oils were characterised by the high amount of monoterpenes (59.1-75.6%), whereas sesquiterpenes were present in smaller quantity (21.2-29.2%). The main compounds in the flower oil were sabinene (18.5%), limonene (12.0%), beta-phellandrene (12.0%) and terpinen-4-ol (10.6%), while in the leaf oil the most dominant were beta-pinene (20.0%) and terpinen-4-ol (12.0%). The antimicrobial activity of the oils was determined using the agar diffusion and broth microdilution method against bacteria Staphylococcus aureus, St. epidermidis, Micrococcus luteus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and a yeast Candida albicans. The best inhibitory effect (MIC 0.6 mg/mL) was exhibited by the flower oil against St. epidermidis, K pneumoniae and C. albicans, the leaf oil against M. luteus, and both oils against E. coli.