In the present study, Monarda didyma L. essential oil (isolated from the flowering aerial part s of the plant) was examined to characterize its chemotype and to evaluate, in addition to the quali-quantitative chemical analysis, the associated antioxidant and antiinflammatory activities.The plants were grown in central Italy, Urbino (PU), Marche region.Different analyses (TLC, GC-FID, GC-MS and 1 H-NMR) allowed to identify twenty compounds among which carvacrol, p-cymene and thymol were the most abundant.On this basis, the chemotype examined in the present study was indicated as Monarda didyma ct.carvacrol.The antioxidant effect was assessed by DPPH assay.Moreover, this chemotype was investigated for the anti-inflammatory effect in an in vitro setting (i.e., LPS-stimulated U937 cells).The decreased expression of pro-inflammatory cytokine IL-6 and the increased expression of miR-146a are suggestive of the involvement of the Toll-like receptor-4 signaling pathway.Although further studies are needed to better investigate the action mechanism/s underlying the results observed in the experimental setting, our findings show that M. didyma essential oil is rich in bioactive compounds (mainly aromatic monoterpenes and phenolic monoterpenes) which are most likely responsible for its beneficial effect.