Abstract The essential oils from wild populations of Mentha longifolia (L.) growing in three distinctive geographic areas of Israel were investigated and compared to the results of earlier studies. The major constituent of all three oils was found to be 1,8-cineole (26.4â34.5%) followed by menthone (10.0â16.7%), pulegone (7.0â7.5%), and isomenthone (4.7â7.8%). Despite some differences in the component proportions, the plants of all three populations clearly belong to the same chemotype, distinct from the previously-studied Mount Sinai and Judean desert varieties of M. longifolia.