The essential oils obtained from leaves and flowers/fruits of Eupatorium cannabinum collected on Amiata Mount (South Tuscany, Italy) were studied by GC and GC/MS. Eighty-three constituents were identified in both oils, the major oxygenated components being methyl thymol (7.8% and 12.2%, respectively) and spathulenol (7.3% and 10.8%, respectively) in the leaf and flower/fruit oils, respectively.