Essential oil (EO) from aerial parts of Eupatorium adenophorum Spreng was extracted by steam distillation and characterized by Gas chromatography (GC) and gas chromatographyâmass spectrometry (GC-MS). Î±-epi-cadinenol (16.63%), O-cymene (13.54%), bornyl acetate (7.70%), Î²-phellandrene (7.46%), and Ï-2-carene (5.77%) were the major terpenoids. The EO showed promising toxicity (median lethal concentration, LC50 = 3176.54 mg Lâ1) and repellent activity (median repellent concentration, RC50 = 2070.99 mg Lâ1) to larvae of Plutella xylostella within 24 h. Among fractions, hexane fraction was more effective to P. xylostella (LC50 = 5056.74 mg Lâ1), whereas methanol fraction to Aphis craccivora (LC50 = 1175.83 mg Lâ1).