Abstract A headspace solvent microextraction (HSME) method for extraction of volatile components of aerial parts of Thymus daenensis subsp. daenensis plant into a microdrop is described. A comparison was made between the results obtained by HSME and a conventional hydrodistillation method. From 36 volatile components found in the hydrodistillated extract, 20 were identified and quantified in the HSME extract. The major components for both isolates were geraniol (34.9â37.2%), geranyl acetate (15.3â18.7%), geranial (9.0â11.2%), nerol (6.4â8.3%) and neral (7.1â9.3%).