Abstract The essential oil of spontaneous plants of Calamintha nepeta (L.) Savi subsp. glandulosa (Req.) P.W. Ball with mint-like character were investigated by GC/MS. Twenty three components were identified in the oil representing 99.7%. The major compounds were pulegone (41.0%), menthone (32.0%), piperitone (7.3%) and piperitenone (7.0%) Key Word Index: Calamintha nepeta subsp. glandulosa Labiataeessential oil compositionpulegonementhone