Abstract The chemical composition of essential oils isolated from fresh flowers and aerial parts of Thymus hirtus Willd was analyzed using GC and GC/MS. Forty-six components were identified in the oil of each organ. Camphor occurred as the major constituent of the aerial part oil, while Î±-pinene was the most notably dominating compound in the flower oil. Biological activities of the indicated oils were evaluated against eight bacteria, nine phytopathogenic, and 15 human pathogenic fungi.