Abstract The differences between the composition of essential oils isolated from seven wild marjoram (Origanum vulgare L. ssp. vulgare) samples, gathered from different regions and cultivated in Estonia were investigated. Steam distillation was used for oil isolation and capillary GC/FID and GC/MS for oil analyses. Forty-eight compounds were identified, representing over 95% of the total oils. Different compositions of essential oils cultivated in Estonia were found. The major compounds of the oils were linalool (0.3â20.6%), Î²-caryophyllene (1.3â45.0%), germacrene D (0.7â21.0%), caryophyllene oxide (1.5â31.3%) and spathulenol (0.9â10.1%)