Mosiera Small is a genus of Myrtaceae family with about forty species identified, including the Cuban endemic plant Mosiera bullata (Britton & P.Wilson) Bisse subsp. bullata. Mosiera species have been little investigated, and this is the first study analysing the composition of Mosiera bullata. Fresh leaf samples of M. bullata were collected from different locations near Santa Clara city, Cuba, and their essential oil was extracted. Using GC/MS, nineteen components (89.68% of the oil) were identified. The major compounds were found to be 1,8-cineole and eugenol, and a GC/FID method was analytically validated for their quantification. Chromatograms (GC/FID) of the sixteen samples studied were proposed as a fingerprint of the volatile composition of M. bullata leaves. Chemometric methods assured the reliability of the chromatographic fingerprinting. Therefore, in the present work is suggested, for the first time, a reliable and consistent chemical profile of M. bullata essential oil.