Volatile oils of the Indian Perilla [Perilla frutescens (L.) Britton], isolated from different phenophases, were analyzed by gas chromatography/flame ionization detector (GC/FID) and GCâmass spectrometry (GC/MS). Essential oil yield was varied from 0.26% to 0.35% during different phenophases of P. frutescens. However, leaves and inflorescence possessed 0.50% and 0.25% essential oil, respectively. Major constituents of the oil were perilla ketone (39.5â59.3%), isoegomaketone (9.4â45.7%), (E)-caryophyllene (0.1â13.4%), and caryophyllene oxide (0.4â3.8%). Perilla ketone was found to be higher at seed maturity stage (59.3%), while the amount of isoegomaketone was recorded higher during vegetative stages (39.8â43.6%). Further, perilla ketone and (E)-caryophyllene were found to be higher in inflorescence, while isoegomaketone was higher in leaves. The investigated P. frutescens chemotype (perilla ketone/isoegomaketone) is new to India.