Abstract The essential oil of Otostegia michauxii Briq. growing wild in the Fars province of Iran was obtained by hydrodistallation and analyzed by GC and GC/MS techniques. Forty-five components representing 97.9% of the total oil were identified. The main components of the oil were dillapiole (23.9%), 2-methylbenzofuran (12.9%) and Î±-pinene (8.1%).