Abstract A water-distilled essential oil from the aerial parts of Johrenia ramosissima Mozaffarian, a newly described endemic species in Iran, was analyzed by GC/MS. Thirty-five compounds representing 88.5% of the oil were identified with bornyl acetate (14.8%), trans-pinocarveol (12.5%) and Î±-campholenol (9.5%) as main constituents.