Abstract The essential oils obtained from air-dried subterranean parts of Valeriana officinalis ssp. collina (Wallr.) Nyman (Valerianaceae), âShipka,â was analyzed by GC and GC/MS, and 62 components were identified. The oil yield, collected in plants grown in 1995 and 1998, was 0.42% (v/m) and 0.40% (v/m) respectively, related to the air-dried roots. Two new sesquiterpenes, (â)-pacifigorgia-l(6),10-diene and (â)-valerena-4,7(11)-diene were identified for the first time in both oils. Some major constituents were bornyl acetate (6.0%-7.3%), spathulenol (5.2%-4.1%), valeranone (12.0%-6.8%), valerenal (9.1%-8.5%), Î±-kessyl acetate (2.1%-6.2%) and (â)-valerena-4,7(11)-diene (7.2%-4.6%).