The aerial parts of essential oils were obtained by hydrodistillation in a modified clevenger-type apparatus and their analysis were performed by HS-SPME. The essential oil aerial parts of Marrubium astracanicum subsp. astracanicum was investigated. Thirty compounds were identified representing 85.5 % of the total components in the oils, respectively. Î²-caryophyllene (23.5 %), germacrene D (10.6 %), spathulenol (9.4 %) and bicyclogermacrene (9.3 %) were identified as major components of M. astracanicum subsp. astracanicum. The disc diffusion method was used to study the antibacterial activity of M. astracanicum subsp. astracanicum essential oil against sixteen bacteria strains. Zone diameter of inhibition from 6 to 13 mm for Gram (+) bacteria. Whereas, for Gram (-) bacteria strains have not shown the response to the essential oil action. In conclusion, this study results might be helpful chemotaxonomy, antimicrobial activity and potential usefulness of Marrubium taxa. Further research is required to evaluate the practical values of therapeutic applications.