Helichrysum italicum ssp. microphyllum extract was isolated by supercritical CO2 extraction with a fractional separation technique. Operative conditions were: extractor, 90 bar and 50°C for 240 min; first separator, 90 bar and -10°C; second separator, 15 bar and 15°C. GC/MS analysis of the leaf volatile concentrate revealed that it mainly consisted of neryl acetate (26.0%), nerol (9.1%), neryl propionate (6.7%), γ-curcumene (10.8%) and cis-dihydro-occidentalol (4.3%). The differences observed between the composition of the SFE volatile concentrate and the hydrodistilled oil were minor.