Abstract The essential oils from leaves of two specimens of Eugenia brasiliensis collected at two locations in the south-eastern Brazilian cerrado were analyzed by GC and GC/MS. The main constituents found in the leaf oil from both specimens were α- and β-selinene and β-caryophyllene. The specimen collected at Jaboticabal contained β-selinene (17.3%) as the major component, while the specimen from Martinho Prado contained α-selinene (14.8%) as the major compound. Additionally, the specimen from Martinho Prado produced relatively high amounts of α- and β-pinene (6.6% and 3.6 %, respectively).