The essential oil of aerial parts of Warionia saharae was obtained by hydrodistillation and analyzed by gas chromatography/flame ionization detector (GC/FID), GC/mass spectrometry (GC/MS) and 13C nuclear magnetic resonance (NMR). In total, forty-three constituents, mainly oxygenated mono- and sesquiterpenes, were identified representing 89.2% of the total oil. Sixteen samples of essential oil of W. saharae collected in three locations exhibited similar chemical compositions showing a chemical homogeneity. The essential oil showed poor antifungal activity against filamentous fungi, Alternaria alternata, Cladosporium herbarum, Fusarium oxysporum and Aspergillus flavus with minimum inhibitory concentration (MIC) values between 4 and 8 Î¼L/mL.