Abstract Water-distilled essential oils from aerial parts of Hymenocrater calycinus (Boiss.) Benth. collected from three different locations of Bojnourd (Iran) village of Yekeh-Shakh (sample A), village of Nodeh (sample B) and Golestan forest (sample C), were analyzed by GC and GC/MS. Î±-Pinene (10.5%) and sabinene (10.5%) were the major constituents of sample A. The main constituents found for sample B were spathulenol (35.4%) and abietatriene (13.4%). In sample C Î²-caryophyllene (32.8%) and caryophyllene oxide (16.1%) were the most abundant compounds.