Artemisia pallens Wall. ex DC, commonly known as davana, is cultivated in India for extraction of its essential oil (DO). In this study, DO collected from thirteen locations (DO1-DO13) and those extracted from six different phenophases and different plant parts were investigated using GC-FID and GC-MS. Altogether, 84 constituents were identified, forming 81.0â96.2% of the total oil composition. Major components of the oils were cis-davanone (17.4â65.1%), bicyclogermacrene (4.0â22.0%), cis-methyl jasmonate (0.4â8.5%), Î²-eudesmol (0.3â6.6%), davana ether isomer (0.5â6.1%), trans-ethyl cinnamate (1.4â6.1%), and germacrene D (0.1â4.7%). Among all tested oils, the DO10, having low cis-davanone content (27.5%), was found more potent against test microorganisms. The oils collected from different locations showed variations in their physical properties, chemical composition, and antimicrobial activity. Based on the yield and cis-davanone content, the flower bud and full-flowering stages could be suitable for harvesting good quality oil in north Indian plains.