Teucrium poliumis widely used in Algerian folk medicine as to treat wounds. The aim of this study was to evaluate the chemical composition, antioxidant and wound healing properties of Teucrium polium essential oil. The composition was obtained by a combination of GC-FID and GC-MS analyses. The antioxidant activity was evaluated by in vitro assays (total antioxidant capacity, DPPH and bleaching of β-carotene). The in vivo wound healing potential of an ointment containing 10% of T. polium essential oil was investigated. The main components were in this order: β-pinene, germacrene, α-pinene, myrcene, limonene, bicyclogermacrene, trans-β-guaiene, spathulenol and β-bourbonene. Teucrium polium essential oil displayed a moderate antioxidant activity. The in vivo experiments showed that 10% OEO accelerated the wound healing process in comparison with controls. This study provides a scientific rationale for the use of Teucrium polium essential oil in the treatments of wounds.