Abstract The volatile constituents of Anthemis altissima L. var. altissima were isolated by hydrodistillation and analyzed by GC and GC/MS. The major constituents of A. altissima flower oil were spathulenol (18.7%), caryophyllene oxide (9.3%), 1-eicosene (7%) and sabinene (6.2%), while the leaf oil contained spathulenol (18.2%), caryophyllene oxide (9.5%), methyl hexadecanoate (8%) and isocaryophyllene (7.4%).