As a result of GC and GC-MS analysis of the essential oils from different parts of Ferulago trifida Boiss., a total of thirty-three compounds were identified in the flowers (Fl), stems (S), leaves (L) and fruits (Fr) essential oils, of which (E)-β-ocimene (Fl: 37.3, S: 20.7, L: 25.7, Fr: 30.5%), α-pinene (Fl: 16.3, S: 22.6, L: 19.6, Fr: 18.0%) and bornyl acetate (Fl: 9.4, S: 8.5, L: 16.7, Fr: 11.0%) were main compounds. Among the thrity-eight compounds identified in the roots essential oil, suberosin (20.7%), β-barbatene (6.6%) and cuparene (6.1%) were main compounds. All essential oils exhibited weak DPPH free radical scavenging activity (IC50: 95â120 µg mlâ1). Among the tested samples flowers, fruits and roots essential oils by 72.1, 78.7 and 74.3% inhibition of Ach Enzyme, respectively, exhibited considerable AChE inhibitory effects in Elman method. They had moderate cytotoxic activity (IC50: 15â55 µg mlâ1) on cancerous cell lines (MCF-7, A-549 and HT-29) in MTT assay.