Abstract The chemical composition of the essential oil obtained from the aerial parts of the cultivated Helichrysum amorginum was investigated by GC and GC/MS. From the 29 identified constituents representing 85.0% of the total oil, spathulenol (36.6%) and Î²-pinene (12.5%) were the major components. Furthermore, it was found that the oil exhibited a moderate antimicrobial activity against four Gram-negative bacteria, two Gram-positive and three pathogenic fungi.