Abstract Commercial Australian sandalwood oil produced from Santalum spicatum (R. Br.) A. DC. roots was analyzed using GC and GC/MS. Seventy constituents were identified: four monoterpenes, 64 sesquiterpenes and two others. Four compounds (Z)-Î²-curcumen-12-ol, (Z)-12-hydroxysesquicineole,6,10-epoxybisabol-2-en-12-olandnor-helifolen-12-al were found to our knowledge for die first time in nature and were characterized using 1H-, 13C-N M R, GC/FTIR and GC/MS analyses.