The antibacterial potential of leaf's essential oil (EO) from Brazilian pepper tree (Schinus terebinthifolius Raddi) against staphylococcal isolates from dogs with otitis externa was evaluated. The minimum inhibitory concentration of EO ranged from 78.1 to 1,250 Î¼g/mL. The oil was analyzed by GC and GC/MS and cytotoxicity tests were carried out with laboratory animals.