Abstract Shoot oil of Tanacetum annuum was examined by GC and GC/MS and found to contain sabinene (22.3%) and camphor (13.2%) as major constituents. This oil was screened for its antifungal activity against Botrytis cinerea, Helminthosporium oryzae, Alternaria solani, Piricularia oryzae and Verticillium dahliae. T. annuum oil exhibited the strongest fungitoxicity by completely inhibiting the mycelial growth of each of the fungi at 5000 ppm.