Abstract The chemical composition of essential oils isolated from leaves of Eucalyptus houseana, E. citriodora, E. raveretiana, E. robusta and E. urophylla collected at N'Débougou (Mali) was determined by gas chromatography (GC) and GC coupled with mass spectrometry (GC/MS). Sixty-seven compounds were identified. The main constituents were α-pinene (1.3â23.9%), p-cymene (0.3â30.6%), 1,8-cineole (12.3â32.1%), γ-terpinene (0.2â22.3%) and citronellal, present only in E. citriodora (76.3%).