A wide number of studies dealing with mosquito control focus on toxicity screenings of whole plant essential oils, while limited efforts shed light on main molecules responsible of toxicity, as well as their mechanisms of action on non-target organisms. In this study, GC-MS shed light on main essential oil components extracted from leaves of the Suriname cherry Eugenia uniflora, i.e., curzerene (35.7%), trans-β-elemenone (11.5%), and γ-elemene (13.6%), testing them on Anopheles subpictus, Aedes albopictus, and Culex tritaeniorhynchus larvae. Non-target toxicity experiments were carried out on four species of aquatic larvivorous organisms, including fishes, backswimmers, and waterbugs. The essential oil from E. uniflora leaves tested on An. subpictus, Ae. Albopictus, and Cx. tritaeniorhynchus showed LC<sub>50</sub> of 31.08, 33.50, and 36.35 μg/ml, respectively. Curzerene, trans-β-elemenone, and γ-elemene were extremely toxic to An. subpictus (LC<sub>50</sub> = 4.14, 6.13, and 10.53 μg/ml), Ae. albopictus (LC<sub>50</sub> = 4.57, 6.74, and 11.29 μg/ml), and Cx. tritaeniorhynchus (LC<sub>50</sub> = 5.01, 7.32, and 12.18 μg/ml). The essential oil from E. uniflora leaves, curzerene, trans-β-elemenone, and γ-elemene showed low toxicity to larvivorous fishes, backswimmers, and waterbugs, with LC<sub>50</sub> ranging from 303.77 to 6765.56 μg/ml. Predator safety factor (PSF) ranged from 55.72 to 273.45. Overall, we believe that curzerene isolated from the essential oil from E. uniflora leaves can represent an ideal molecule to formulate novel mosquito larvicides, due to its extremely low LC<sub>50</sub> on all tested mosquito vectors (4.14-5.01 μg/ml), which far encompasses most of the botanical pesticides tested till now. Notably, the above-mentioned LC<sub>50</sub> did not damage the four aquatic predators tested in this study.