The composition of the volatile oils from the aerial parts of Hypericum perforatum L. collected in six localities from southeastern France was analysed by GC-MS. Twenty-nine to 41 compounds have been identified in these volatile oils. The main constituents were sesquiterpene hydrocarbons, and minor variations were pointed out in the oil composition among the six populations. However, the composition of all the analysed oils greatly varied from that of the previous studies, carried out on H. perforatum essential oils from other localities, in which monoterpenoids were the major constituents, particularly, the alpha-pinene.