The essential oil composition and biological activities of Chrysanthemum coronarium L. from three locations in Cyprus were investigated. A total of 35 components were identified in the oils representing 86.2 ± 0.7%â93.8 ± 0.0% (n = 3) of the oils. Camphor 25.2 ± 0.1%â9.1 ± 0.3%, santolinatriene 21.7 ± 0.1%â3.6 ± 0.1%, yomogi alcohol 12.6 ± 0.1%â3.6 ± 0.1%, cis-chrysanthenyl acetate 10.7 ± 0.1%â6.8 ± 0.1% and bornyl acetate 11.0 ± 0.5%â5.7 ± 0.0% were identified as the main components. Highest DPPH scavenging and PRAP activity was observed for the LefkoÅe sample (49.59 ± 0.19% (n = 5) and 812.77 ± 4.34 AU (n = 5)). Highest phytotoxic activity against Lemna minor L. was observed for the Salamis sample (100.0%). LefkoÅe sample showed the highest insecticidal activity (97.82 ± 1.79% mortality, S. granarius). Bornyl acetate/cis-chrysanthenyl acetate/hexadecanoic acid and camphor/santolinatriene/ yomogi alcohol chemotypes of C. coronarium are proposed from Cyprus samples according to cluster analysis of the oils together with the literature data.