Abstract The chemical composition of the essential oil of the aerial part of Nepeta pannonica L. from Kazakhstan was investigated by GC and GC/MS. Sixty components were characterized from N. pannonica with 1,8-cineole (28.9%), and 4aa,7Î²,7aÎ±-nepetalactone (14.3%) as the major constituents. The oil of N. pannonica was more phytotoxic to bentgrass compared to lettuce seeds, with 100% growth inhibition observed at 0.3, and 1.0 mg/mL, respectively.