Abstract The antifungal activity of the oil of Salvia pomifera ssp. calycina and its main components (α- and β-thujone) were tested on six phytopathogenic fungal strains. The hydrocarbon and oxygenated fractions of the oil were assessed against Rhizoctonia solani and Sclerotinia sclerotiorum, the two most susceptible fungi to the oil tested. The oil exhibited fungistatic effect at the concentration of 1000 μL/L on R. solani and S. sclerotiorum, whereas its oxygenated fraction showed fungicidal activity at 2000 and 1000 μL/L, respectively. The hydrocarbon fraction had a weak effect on the test organisms. The oxygenated monoterpenes α- and β-thujone exhibited a moderate fungistatic effect to R. solani and S. sclerotiorum, while their inhibitory activity was weak to Verticillium dahliae and the Fusarium species tested. Key Word Index: Salvia pomifera ssp. calycina Labiataeessential oil compositionα-thujoneβ-thujoneantifungal activitysoil borne pathogens