The present study aimed to investigate the chemical composition and antimicrobial activity of Jasminum grandiflorum L. essential oil produced for the first time at industrial level by steam distillation. Volatile and chiral compositions were analyzed by GC-MS and GC-FID. In total 130 volatile compounds were identified, which constituted more than 98% of the oils. The results revealed significant differences in terms of volatile compounds between jasmine essential oil and the absolute. Sensorial evaluation revealed the essential oil had a youthful and dynamic character with a bright, floral scent, while the absolute had a more mature and sensual scent that was richer. Antimicrobial activity of both products against eighteen human pathogens and cosmetic contaminants was evaluated. The results showed that essential oil exhibited greater antimicrobial activity, inhibiting 8 out of 17 microbial strains within the MIC range â¤800 µg/mL, compared to 2 out of 17 in the case of the absolute.