Abstract The essential oil obtained by hydrodistillation of the leaves and stems of Eucalyptus globulus Labill ssp. bicostata from Jujuy, in the northwest of Argentina, was analyzed by capillary GC and GC/MS. The headspace of the oil and of the crushed fresh leaves were also analyzed by capillary GC. The main component was 1,8-cineole in all the samples analyzed. Other notable constituents were Î±-pinene, Î±-terpineol, globulol and aromadendrene.