chemical investigation of the leaves and fresh flowers of lonicera japonica lead to the isolation of a new pentacyclic triterpene saponin, 3- o-[-l-rhamnopyranosyl-(1->2)--d-xylopyranosyl]-hedragenin (1) along with two known saponins, 3- o-[-l-arabinopyranosyl]-hedragenin (2) and 3- o-[-l-rhamnopyranosyl-(1->2)--l-arabinopyranosyl]-hedragenin (3), two fatty acid esters, methyl (z,z) 9,12-octadecadienoate (methyl linoleate) (4) and methyl (z,z,z) 9,12,15-octadecatrienoate (methyl linolineate) (5) and two long chain hydrocarbons, octadecane (6) and heneicosane (7). the structures were established on the basis of ir, 1d- and 2d-nmr (hmqc and hmbc) spectroscopic and esi-qtof-ms/ms analysis. simultaneously, the volatile composition of fresh flowers of l. japonica, analyzed by gc/ms, indicated the presence of two long chain hydrocarbons, heneicosane (37.5%) and octadecane (34.5%) as major constituents. other metabolites characterized were fatty acids and their esters, such as palmitic acid (1.7%), linolenic acid (2.5 %), linoleic acid (1.3%), methyl palmitate (2.3%), methyl 9,12-octadecadienoate (4.5%) and methyl ( z,z,z)-9,12,15-octadecatrienoate, along with oxygenated monoterpenes and sesquiterpenes, namely linalool, cis-linalool oxide, terpine-4-ol and trans-nerolidol.