abstract the alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree nicotiana glauca graham (solanaceae) was analyzed using uplc-ms and gc-ms. anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % ( m / m ) of the alkaloid-rich fraction. in addition to anabasine, six secondary metabolites were identified using high-resolution uplc-ms. anabasine was quantified in the leaves to be 1 mg g -1 dry plant material. the gc-ms analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. moreover, gc-ms was used for the analysis of the volatile oil that was obtained by hydro-distillation from the leaves of n. glauca . the volatile plant oil was found to be rich in oxygenated sesquiterpenes ( e.g ., -bisabolol) and carboxylic acids and esters ( e.g ., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected.