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Title insecticidal and histopathological effects of ageratum conyzoides weed extracts against dengue vector, aedes aegypti
Journal insects
Year 2020
DOI 10.3390/insects11040224
Cited By 19
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Abstract
crude extracts and essential oils of a. conyzoides were tested with larva and adult stages of ae. aegypti mosquitoes to determine their insecticidal properties. the crude extracts and essential oils came from three varieties of a. conyzoides (with white flowers, purple flowers, or white-purple flowers) and from two places on each plant (leaves and flowers), giving six types overall: leaf-white (lw); leaf-purple (lp); leaf white-purple (lw-p); flower-white (fw); flower-purple (fp); and flower white-purple (fw-p). chemical constituents and components of the essential oils were identified using gas chromatography-mass spectrometry (gc-ms). electron microscopic and histopathological studies were performed to determine the toxicological effects on mosquitoes in terms of morphological alterations. the six types of crude extracts exhibited no activity against individuals in the larval stages. however, six types of essential oils were effective against adult ae. aegypti females. the mortality of adult ae. aegypti females was higher from leaf extracts, particularly lp (median lethal dose, ld50 = 0.84%). the number of chemical constituents identified by gc-ms was high in flowers, especially w-p. precocene i was the most abundant chemical component among the five types of essential oils, except in lp, in which precocene ii was the most abundant. histopathological alterations in adult ae. aegypti females included compound eye degeneration, muscular damage with cellular infiltration, gut epithelial degeneration and necrosis, pyknotic nuclei in the malpighian epithelium and ovarian cell degeneration. fw and fp plant types exhibited the highest severity of histopathological alterations in mosquitoes compared with other plants, probably owing to the presence of monoterpene compounds in their tissues. the present study demonstrated lp plant extracts from a. conyzoides could be effective adulticides against adult ae. aegypti. as natural products are biodegradable and exhibit low toxicity to mammalian and non-target organisms, they are suitable candidates for use in vector control programmes.
1
Plants
15
Compounds
126
Records

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Compounds
Plants
Plant Parts
All Records
CompoundCount
beta-caryophyllene12
alpha-caryophyllene12
precocene i12
germacrene d12
copaene12
caryophyllene oxide12
precocene ii11
beta-pinene6
alpha-pinene6
limonene6
6-vinyl-7-methoxy-2 2-dimethylchromene6
camphene5
beta-bourbonene5
6-vinyl-7-methoxy-2,2-dimethylchromene5
endo-bornyl acetate4
PlantFamily
Ageratum conyzoidesasteraceae
Plant PartCount
flower71
leaf55
PlantCompoundPart%
Ageratum conyzoides 6-vinyl-7-methoxy-2 2-dimethylchromene leaf 0.17
Ageratum conyzoides 6-vinyl-7-methoxy-2 2-dimethylchromene leaf 0.39
Ageratum conyzoides 6-vinyl-7-methoxy-2 2-dimethylchromene flower 0.17
Ageratum conyzoides 6-vinyl-7-methoxy-2 2-dimethylchromene flower 0.17
Ageratum conyzoides 6-vinyl-7-methoxy-2 2-dimethylchromene leaf 0.28
Ageratum conyzoides 6-vinyl-7-methoxy-2 2-dimethylchromene flower 0.28
Ageratum conyzoides 6-vinyl-7-methoxy-2,2-dimethylchromene leaf 0.17
Ageratum conyzoides 6-vinyl-7-methoxy-2,2-dimethylchromene leaf 0.28
Ageratum conyzoides 6-vinyl-7-methoxy-2,2-dimethylchromene flower 0.17
Ageratum conyzoides 6-vinyl-7-methoxy-2,2-dimethylchromene leaf 0.39
Ageratum conyzoides 6-vinyl-7-methoxy-2,2-dimethylchromene flower 0.28
Ageratum conyzoides alpha-caryophyllene flower 5.09
Ageratum conyzoides alpha-caryophyllene leaf 2.51
Ageratum conyzoides alpha-caryophyllene flower 6.62
Ageratum conyzoides alpha-caryophyllene leaf 1.39
Ageratum conyzoides alpha-caryophyllene flower 4.09
Ageratum conyzoides alpha-caryophyllene leaf 1.39
Ageratum conyzoides alpha-caryophyllene leaf 2.36
Ageratum conyzoides alpha-caryophyllene flower 4.09
Ageratum conyzoides alpha-caryophyllene flower 5.09
Ageratum conyzoides alpha-caryophyllene leaf 2.36
Ageratum conyzoides alpha-caryophyllene leaf 2.51
Ageratum conyzoides alpha-caryophyllene flower 6.62
Ageratum conyzoides alpha-pinene flower 0.06
Ageratum conyzoides alpha-pinene flower 0.12
Ageratum conyzoides alpha-pinene flower 0.12
Ageratum conyzoides alpha-pinene flower 0.1
Ageratum conyzoides alpha-pinene flower 0.1
Ageratum conyzoides alpha-pinene flower 0.06
Ageratum conyzoides beta-bourbonene leaf 0.12
Ageratum conyzoides beta-bourbonene leaf 0.12
Ageratum conyzoides beta-bourbonene leaf 0.14
Ageratum conyzoides beta-bourbonene leaf 0.14
Ageratum conyzoides beta-bourbonene leaf 0.12
Ageratum conyzoides beta-caryophyllene leaf 24.17
Ageratum conyzoides beta-caryophyllene leaf 20.09
Ageratum conyzoides beta-caryophyllene leaf 24.17
Ageratum conyzoides beta-caryophyllene leaf 20.6
Ageratum conyzoides beta-caryophyllene leaf 20.6
Ageratum conyzoides beta-caryophyllene flower 22.86
Ageratum conyzoides beta-caryophyllene leaf 20.09
Ageratum conyzoides beta-caryophyllene flower 22.86
Ageratum conyzoides beta-caryophyllene flower 24.67
Ageratum conyzoides beta-caryophyllene flower 21.49
Ageratum conyzoides beta-caryophyllene flower 24.67
Ageratum conyzoides beta-caryophyllene flower 21.49
Ageratum conyzoides beta-pinene flower 0.11
Ageratum conyzoides beta-pinene flower 0.06
Ageratum conyzoides beta-pinene flower 0.05
Ageratum conyzoides beta-pinene flower 0.05
Ageratum conyzoides beta-pinene flower 0.06
Ageratum conyzoides beta-pinene flower 0.11
Ageratum conyzoides camphene flower 0.93
Ageratum conyzoides camphene flower 0.93
Ageratum conyzoides camphene flower 0.93
Ageratum conyzoides camphene flower 0.58
Ageratum conyzoides camphene flower 0.58
Ageratum conyzoides caryophyllene oxide leaf 0.87
Ageratum conyzoides caryophyllene oxide leaf 0.99
Ageratum conyzoides caryophyllene oxide flower 0.43
Ageratum conyzoides caryophyllene oxide flower 0.58
Ageratum conyzoides caryophyllene oxide flower 0.45
Ageratum conyzoides caryophyllene oxide leaf 0.58
Ageratum conyzoides caryophyllene oxide leaf 0.58
Ageratum conyzoides caryophyllene oxide flower 0.45
Ageratum conyzoides caryophyllene oxide leaf 0.99
Ageratum conyzoides caryophyllene oxide flower 0.43
Ageratum conyzoides caryophyllene oxide leaf 0.87
Ageratum conyzoides caryophyllene oxide flower 0.58
Ageratum conyzoides copaene leaf 0.2
Ageratum conyzoides copaene flower 0.37
Ageratum conyzoides copaene flower 0.08
Ageratum conyzoides copaene leaf 0.11
Ageratum conyzoides copaene leaf 0.21
Ageratum conyzoides copaene flower 0.08
Ageratum conyzoides copaene leaf 0.11
Ageratum conyzoides copaene flower 0.37
Ageratum conyzoides copaene leaf 0.21
Ageratum conyzoides copaene leaf 0.2
Ageratum conyzoides copaene flower 0.52
Ageratum conyzoides copaene flower 0.52
Ageratum conyzoides endo-bornyl acetate flower 0.55
Ageratum conyzoides endo-bornyl acetate leaf 0.17
Ageratum conyzoides endo-bornyl acetate leaf 0.17
Ageratum conyzoides endo-bornyl acetate flower 0.55
Ageratum conyzoides germacrene d flower 3.79
Ageratum conyzoides germacrene d flower 3.46
Ageratum conyzoides germacrene d flower 3.46
Ageratum conyzoides germacrene d flower 3.79
Ageratum conyzoides germacrene d flower 5.64
Ageratum conyzoides germacrene d flower 5.64
Ageratum conyzoides germacrene d leaf 1.48
Ageratum conyzoides germacrene d leaf 2.87
Ageratum conyzoides germacrene d leaf 2.87
Ageratum conyzoides germacrene d leaf 1.48
Ageratum conyzoides germacrene d leaf 2.98
Ageratum conyzoides germacrene d leaf 2.98
Ageratum conyzoides limonene flower 0.34
Ageratum conyzoides limonene flower 0.14
Ageratum conyzoides limonene flower 0.14
Ageratum conyzoides limonene flower 0.34
Ageratum conyzoides limonene flower 0.18
Ageratum conyzoides limonene flower 0.18
Ageratum conyzoides precocene i leaf 48.04
Ageratum conyzoides precocene i flower 36.15
Ageratum conyzoides precocene i leaf 61.32
Ageratum conyzoides precocene i flower 42.86
Ageratum conyzoides precocene i leaf 48.04
Ageratum conyzoides precocene i flower 25.76
Ageratum conyzoides precocene i flower 42.86
Ageratum conyzoides precocene i leaf 13.99
Ageratum conyzoides precocene i leaf 13.99
Ageratum conyzoides precocene i leaf 61.32
Ageratum conyzoides precocene i flower 36.15
Ageratum conyzoides precocene i flower 25.76
Ageratum conyzoides precocene ii flower 15.96
Ageratum conyzoides precocene ii leaf 12.81
Ageratum conyzoides precocene ii leaf 0.87
Ageratum conyzoides precocene ii leaf 12.81
Ageratum conyzoides precocene ii flower 14.45
Ageratum conyzoides precocene ii flower 15.96
Ageratum conyzoides precocene ii leaf 47.49
Ageratum conyzoides precocene ii flower 14.45
Ageratum conyzoides precocene ii leaf 0.87
Ageratum conyzoides precocene ii flower 14.45
Ageratum conyzoides precocene ii leaf 47.49