th e chemical composition of the essential oil obtained from the fruits of pimpinella affi nis ledeb. (apiaceae) was analyzed by gas chromatography (gc) and gas chromatography - mass spectrometry (gc-ms) techniques. twenty - four components were identifi ed in the essential oil of p. affi nis ledeb., whose major constituents were geijerene (17.68%), limonene (12.86%), pregeijerene (9.92%), germacrene d (8.54%) and trans- -ocimene (4.94%). th e essential oil was evaluated for antibacterial and antifungal activities. th e oil showed antimicrobial activity against all the tested microorganisms, excepted pseudomonas aeruginosa. maximum activity was observed against fungal microorganisms. key words: antimicrobial; apiaceae, essential oil, pimpinella affi nis ledeb