objectives: artemisia scoparia is a perennial herb belonging to the asteraceae family. although research has shown that a. scoparia possesses antioxidant and anti-inflammatory properties, the anti-inflammatory activity and mechanisms of a. scoparia essential oil (aseo) remain poorly understood. methods: anti-inflammatory activities and mechanisms of aseo were investigated using cellular experiments, gas chromatography-mass spectrometry (gc-ms), network pharmacology, and molecular docking. key findings: cellular assays showed that aseo ameliorated lipopolysaccharide (lps)-induced inflammation in raw264.7 cells by inhibiting nitric oxide (no), myeloperoxidase (mpo), and tumor necrosis factor alpha (tnf-) production and thereby suppressing inflammation in a dose-dependent manner. notably, the effect of aseo (6.25 g/ml) was stronger than that of the positive control dexamethasone (7.85 g/ml). the network pharmacology and molecular docking results indicated that the key components (methyleugenol, l--terpineol, -bisabolol, and -cadinol) exerted significant anti-inflammatory effects by acting on the key targets [peroxisome proliferative activated receptor gamma (pparg), prostaglandin-endoperoxide synthase 2 (ptgs2), estrogen receptor 1 (esr1), e1a binding protein p300 (ep300), peroxisome proliferator activated receptor alpha (ppara), and 3-hydroxy-3-methylglutaryl-coa reductase (hmgcr)], and the main pathways involved were mainly the ppar signaling pathway, neuroactive ligand-receptor interaction, cyclic adenosine monophosphate (camp) signaling pathway, and serotonergic synapse. conclusions: these findings suggest that aseo has great potential as a natural anti-inflammatory agent, its key compositions and multiple anti-inflammatory mechanisms make it a promising candidate for further research and development for clinical applications.