salvia sclarea essential oil (sseo) has a long tradition in the food, cosmetic, and perfume industries. the present study aimed to analyze the chemical composition of sseo, its antioxidant activity, antimicrobial activity in vitro and in situ, antibiofilm, and insecticidal activity. besides that, in this study, we have evaluated the antimicrobial activity of sseo constituent (e)-caryophyllene and standard antibiotic meropenem. identification of volatile constituents was performed by using gas chromatography (gc) and gas chromatography/mass spectrometry (gc/ms) techniques. results obtained indicated that the main constituents of sseo were linalool acetate (49.1%) and linalool (20.6%), followed by (e)-caryophyllene (5.1%), p-cimene (4.9%), a-terpineol (4.9%), and geranyl acetate (4.4%). antioxidant activity was determined as low by the means of neutralization of the ddph radical and abts radical cation. the sseo was able to neutralize the dpph radical to an extent of 11.76 +/- 1.34%, while its ability to decolorize the abts radical cation was determined at 29.70 +/- 1.45%. preliminary results of antimicrobial activity were obtained with the disc diffusion method, while further results were obtained by broth microdilution and the vapor phase method. overall, the results of antimicrobial testing of sseo, (e)-caryophyllene, and meropenem, were moderate. however, the lowest mic values, determined in the range of 0.22-0.75 ug/ml for mic50 and 0.39-0.89 ug/ml for mic90, were observed for (e)-caryophyllene. the antimicrobial activity of the vapor phase of sseo (towards microorganisms growing on potato) was significantly stronger than that of the contact application. biofilm analysis using the maldi tof ms biotyper showed changes in the protein profile of pseudomonas fluorescens that showed the efficiency of sseo in inhibiting biofilm formation on stainless-steel and plastic surfaces. the insecticidal potential of sseo against oxycarenus lavatera was also demonstrated, and results show that the highest concentration was the most effective, showing insecticidal activity of 66.66%. the results obtained in this study indicate the potential application of sseo as a biofilm control agent, in the shelf-life extension and storage of potatoes, and as an insecticidal agent.