abstract coriander ( coriandrum sativum l.) is an annual herb belonging to the apiaceae family that is grown worldwide. this aromatic herb has been used for its nutritional value and biological properties. in this study, we compared the essential oil composition and antibacterial activity of coriander seeds from nine iranian and iraqi populations for the first time. the seed oils were extracted using a clevenger-type apparatus, and their chemical composition was determined using gc and gc/ms agilent apparatuses. the antimicrobial activity of the oils was tested against three infectious bacteria ( staphylococcus aureus , escherichia coli , and pseudomonas aeruginosa ) using the agar well diffusion method. the experiments were repeated three times, and the results were analyzed using past, sas, and spss software. the results showed that oxygenated monoterpenes, especially linalool, were the major compounds in the oils, followed by -pinene, -terpinene, and geranyl acetate. the proportions of these compounds varied among the populations. trace amounts of other compounds were also detected, some of which were only found in certain populations. the populations were detected as linalool chemotype, and classified into four groups based on their chemical constituents in the upgma tree. the pca-biplot showed that these groups were characterized by the presence and percentage of specific compounds. the essential oils showed bacterial growth inhibitory properties only at 100% concentration. s. aureus was the most sensitive bacterium to the coriander essential oil, while the essential oils of all populations inhibited the growth of this bacterium. additionally, the essential oils were more effective than antibiotics against e. coli . these findings contribute to our understanding of coriander seed essential oil by providing data on antibacterial activity and chemical characteristics. furthermore, the study highlights the importance of selecting populations based on their specific essential oil profiles for antibacterial applications.